Characterization of Hydrogen Peroxide-Induced DNA Release by Streptococcus sanguinis and Streptococcus gordonii

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Characterization of hydrogen peroxide-induced DNA release by Streptococcus sanguinis and Streptococcus gordonii.

Extracellular DNA (eDNA) is produced by several bacterial species and appears to contribute to biofilm development and cell-cell adhesion. We present data showing that the oral commensals Streptococcus sanguinis and Streptococcus gordonii release DNA in a process induced by pyruvate oxidase-dependent production of hydrogen peroxide (H(2)O(2)). Surprisingly, S. sanguinis and S. gordonii cell int...

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Streptococcal antagonism in oral biofilms: Streptococcus sanguinis and Streptococcus gordonii interference with Streptococcus mutans.

Biofilms are polymicrobial, with diverse bacterial species competing for limited space and nutrients. Under healthy conditions, the different species in biofilms maintain an ecological balance. This balance can be disturbed by environmental factors and interspecies interactions. These perturbations can enable dominant growth of certain species, leading to disease. To model clinically relevant i...

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Streptococcus gordonii Hsa environmentally constrains competitive binding by Streptococcus sanguinis to saliva-coated hydroxyapatite.

Competition between pioneer colonizing bacteria may determine polymicrobial succession during dental plaque development, but the ecological constraints are poorly understood. For example, more Streptococcus sanguinis than Streptococcus gordonii organisms are consistently isolated from the same intraoral sites, yet S. gordonii fails to be excluded and survives as a species over time. To explain ...

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Sphingosines affect the adherence of Streptococcus gordonii and Streptococcus sanguinis on hydroxyapatite

Sphingosines affect the adherence of Streptococcus gordonii and Streptococcus sanguinis on hydroxyapatite. Abstract Sphingosines exhibit bactericidal activity on planktonic cells. However, their anti-adherence activity when adsorbed on hydroxyapatite (HA) surfaces is unknown. Therefore this study evaluated the anti-adherence activity of a variety of sphingosines and their derivatives against St...

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Streptococcus sanguinis induces neutrophil cell death by production of hydrogen peroxide

Streptococcus is the dominant bacterial genus in the human oral cavity and a leading cause of infective endocarditis. Streptococcus sanguinis belongs to the mitis group of streptococci and produces hydrogen peroxide (H2O2) by the action of SpxB, a pyruvate oxidase. In this study, we investigated the involvement of SpxB in survival of S. sanguinis in human blood and whether bacterial H2O2 exhibi...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 2009

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.00906-09